Publications de l'équipe QCPS


Outil Génétique pour l’Etude de l’Activité trans-Traductionnelle et son Utilisation dans le Développement de Nouveaux Antibiotiques.
Macé K, Demay F, Giudice E, Blanco C, Gillet R.
Brevet n°EP16200144

Reassembling GFP to evaluate trans-translation in vitro.
Guyomar C. et Gillet R.
Brevet n° EP18063780

Biofilm prevention, inhibition and controlling process (antibiofilm) using natural or synthetic peptides derived from 2S-sulfur-rich seed storage protein 2-like protein or their peptidomimetic (synthetic) derivatives.
Gomes Von Borowski R. et al.
Brevet nº EP19305205.7

Publications :

> 2023

Structural insights into the binding of bS1 to the ribosome.
D'Urso G., Chat S., Gillet R., Giudice E.
Nat Commun. 2023 Fev.

Purification and Characterization of Authentic 30S Ribosomal Precursors Induced by Heat Shock.
Giudice E., Georgeault S., Pineau C., Trautwetter A., Ermel G., Blanco C., Gillet R.
International Journal of Molecular Sciences. 2023 Fev.


> 2022

Mutation of the proline P81 into a serine modifies the tumour suppressor function of the von Hippel–Lindau gene in the ccRCC.
Chesnel F., Jullion E., Delalande O., Couturier A., Alusse A., Le Goff X., Lenglet M., Gardie B., Abadie C., Arlot-Bonnemains Y.
British Journal of Cancer. 2022 Sept.

Interactions of organophosphorus pesticides with ATP-binding cassette (ABC) drug transporters.
Chedik L, Mias-Lucquin D, Fardel O, Delalande O, Bruyere A.
Xenobiotica. 2022 Jun.

Substrate recognition and cryo-EM structure of the ribosome- bound TAC toxin of Mycobacterium tuberculosis.
Mansour M, Giudice E, Xu X, Akarsu H, Bordes P, Guillet V, Bigot DJ, Slama N, D’Urso G, Chat S, Redder P, Falquet L, Mourey L, Gillet R, Genevaux P.
Nature Commun. 2022 May.

Insights into the ribosomal trans-translation rescue system: lessons from recent structural studies.
D’Urso G, Guyomar C, Chat S, Giudice E, Gillet R.
FEBS Journal. 2022 Jan.


> 2021

Safe and easy in vitro evaluation of tmRNA-SmpB-mediated trans-translation from ESKAPE pathogenic bacteria. .
Thépaut M, Campos-Silva R, Renard E, Barloy-Hubler F, Ennifar E, Boujard D, Gillet R. .
RNA. 2021 Nov.

Capsicumicine, a New Bioinspired Peptide from Red Peppers Prevents Staphylococcal Biofilm In Vitro and In Vivo via a Matrix Anti-Assembly Mechanism of Action. .
Gomes Von Borowski R, Chat S, Schneider R, Nonin-Lecomte S, Bouaziz S, Giudice E, Rigon Zimmer A, Baggio Gnoatto SC, Macedo AJ, Gillet R.
Microbiol Spectr. 2021 Oct.

Structures of tmRNA and SmpB as they transit through the ribosome. .
Guyomar C, D'Urso G, Chat S, Giudice E, Gillet R.
Nat Commun. 2021 Aug.

Reflux of Endoplasmic Reticulum proteins to the cytosol inactivates tumor suppressors. .
Sicari D, Centonze FG, Pineau R, Le Reste PJ, Negroni L, Chat S, Mohtar MA, Thomas D, Gillet R, Hupp T, Chevet E, Igbaria A.
EMBO Rep. 2021 May.


> 2020

Pseudonajide is a short peptide antibiotic derived from snake venom that acts at low concentration interfering with cell wall and inhibiting biofilm.
Schneider R, Primon-Barros M, Gomes Von Borowski R, Chat S, Nonin-Lecomte S, Gillet R, Macedo AJ.
BMC Microbiology. 2020 Aug.

Reassembling green fluorescent protein for in vitro evaluation of trans-translation.
Guyomar C, Thepault M, Nonin-Lecomte S, Mereau A, Goude R, Gillet R.
Nucleic Acids Res. 2020 Feb.

Red pepper peptide coatings control Staphylococcus epidermidis adhesion and biofilm formation.
Gomes Von Borowski R, Primon Barrosa M, Brentan da Silvac D, Peporine Lopesc N, Rigon Zimmerb K, Staatsb CC, Bernardes de Oliveiraa C, Giudice E, Gillet R, Macedo AJ, Baggio Gnoatto SC, Rigon Zimmer A.
Int J Pharm. 2020 Jan.


> 2019

Synthesis and evaluation of 1,3,4-oxadiazole derivatives for development as broad-spectrum antibiotics.
Tresse C, Radigue R, Gomes Von Borowski R, Thepaut M, Le HH, Demay F, Georgeault S, Dhalluin A, Trautwetter A, Ermel G, Blanco C, Van De Weghe P, Jean M, Giard JC, Gillet R.
Bioorg Med Chem. 2019 Nov.

When transfer-messenger RNA scars reveal its ancient origins.
Guyomar C. and R Gillet R.
Ann NY Acad Sci 2019 Jul.

Numerous cultivated and uncultivated viruses encode ribosomal proteins.
Mizuno* CM, Guyomar* C, Roux R, Lavigne R, Rodriguez-Valera F, Sullivan MB, Gillet R, Forterre P, Krupovic M.
Nature Comm. 2019 Fev.

Characterization of HicAB toxin-antitoxin module of Sinorhizobium meliloti.
Thomet M, Trautwetter A, Ermel G, Blanco C.
BMC Microbiol. 2019 Jan.


> 2018

Structural and Functional Organization of Ribosomal Compartment in the Cell.
Karpova EA. and Gillet R.
Trends Biochem Sci. 2018 Dec.

Promising Antibiofilm Activity of Peptidomimetics.
Gomes Von Borowski R, Baggio Gnoatto SC, Macedo AJ, Gillet R.
Front Microbiol. 2018 Sep.

Emerging fields in chaperone proteins: A French workshop.
Mileo E, Ilbert M, Barducci A, Bordes P, Castanié-Cornet MP, Garnier C, Genevaux P, Gillet R, Goloubinoff P, Ochsenbein F, Richarme G, Iobbi-Nivol C, Giudici-Orticoni MT, Gontero B, Genest O.
Biochimie. 2018 Aug.

The structure of an elongation factor G-ribosome complex captured in the absence of inhibitors.
Macé K, Giudice E, Chat S, Gillet R.
Nucleic Acids Res. 2018 Apr.

Dystrophin's central domain forms a complex filament that becomes disorganized by in-frame deletions.
Delalande O, Molza AE, Dos Santos Morais R, Chéron A, Pollet É, Raguenes-Nicol C, Tascon C, Giudice E, Guilbaud M, Nicolas A, Bondon A, Leturcq F, Férey N, Baaden M, Perez J, Roblin P, Piétri-Rouxel F, Hubert JF, Czjzek M, Le Rumeur E.
J Biol Chem. 2018 May.


> 2017

A genetic tool to quantify trans-translation activity in vivo.
Macé K, Demay F, Guyomar C, Georgeault S, Giudice E, Goude R, Trautwetter A, Ermel G, Blanco C, Gillet R.
J Mol Biol, 2017 Nov.

The double life of the ribosome: when its protein folding activity supports prion propagation.
Voisset C, Blondel M, Jones GW, Friocourt G, Stahl G, Chedin S, Beringue V, Gillet R.
Prion. 2017 Mar.

Purification, identification, and functional analysis of polysomes from the human pathogen Staphylococcus aureus.
Brielle R, Pinel-Marie Ml, Chat S, Gillet R, Felden B.
Methods. 2017 Mar.

How High Concentrations of Proteins Stabilize the Amorphous State of Calcium Orthophosphate: A Solid-State Nuclear Magnetic Resonance (NMR) Study of the Casein Case
De Sa Peixoto P, Silva JV, Laurent G, Schmutz M, Thomas D, Bouchoux A, Gésan-Guiziou G.
Langmuir. 2017 Fev.

VLITL is a major cross-β-sheet signal for fibrinogen Aα-chain frameshift variants.
Garnier C, Briki F, Nedelec B, Le Pogamp P, Dogan A, Rioux-Leclercq N, Goude R, Beugnet C, Martin L, Delpech M, Bridoux F, Grateau G, Doucet J, Derreumaux P, Valleix S.
Blood. 2017 Dec.


> 2016

Hsp90 directly interacts, in vitro, with amyloid structures and modulates their assembly and disassembly.
Schirmer C, Lepvrier E, Duchesne L, Decaux O, Thomas D, Delamarche C, Garnier C.
Biochim Biophys Acta. 2016 Nov.

Origins of tmRNA: the missing link in the birth of protein synthesis?
Macé K and Gillet R.
Nucleic Acids Res. 2016 Sep.

Protein Folding Activity of the Ribosome is involved in Yeast Prion Propagation.
Blondel M, Soubigou F, Evrard J, Nguyen Ph, Hasin N, Chédin S, Gillet R, Contesse Ma, Friocourt G, Stahl G, Jones Gw, Voisset C.
Sci Rep. 2016 Sep.

Bacterial Community Diversity Harboured by Interacting Species.
Bili M, Cortesero AM, Mougel C, Gauthier JP, Ermel G, Simon JC, Outreman Y, Terrat S, Mahéo F, Poinsot D.
PLoS One. 2016 Jun.


> 2015

Mechanism of eIF6 release from the nascent 60S ribosomal subunit.
Weis F, Giudice E, Churcher M, Jin L, Hilcenko C, Wong CC, Traynor D, Kay RR, Warren AJ.
Nat. Struct. Mol. Biol. 2015 Nov.

Hsp90 oligomerization process: How can p23 drive the chaperone machineries?
Lepvrier E, Nigen M, Moullintraffort L, Chat S, Allegro D, Barbier P, Thomas D, Nazabal A, Garnier C.
Biochim Biophys Acta. 2015 Oct.

Hsp90 Oligomers Interact with the Aha1 Co-Chaperone: A new Outlook for the Hsp90 Chaperone Machineries.
Lepvrier E, Moullintraffort L, Nigen M, Goude R, Allegro D, Barbier P, Peyrot V, Thomas D, Nazabal A, Garnier C.
Anal Chem. 2015 Jul.

Quality control in protein synthesis. 
Gillet R.
Biochimie. 2015 Jul.
>> Journal cover

La synthèse des protéines par le ribosome : un chemin semé d’embûches [Protein synthesis by the ribosome: a pathway full of pitfalls].
Macé K, Giudice E, Gillet R.
Med Sci (Paris). 2015 Mar.

Diffusion and partitioning of macromolecules in casein microgels: evidence for size-dependent attractive interactions in a dense protein system.
De Sa Peixoto P, Bouchoux A, Huet S, Madec MN, Thomas D, Floury J,  Gesan-Guiziou G.
Langmuir. 2015 Feb.

Recrystallized S Layer protein of a probiotic propionibacterium: structural and nanomechanical changes upon temperature or pH shifts probed by solid-state NMR and AFM.
De Sa Peixoto P, Roiland C, Thomas D, Briard-Bion V, Le Guellec R, Parayre S, Deutsch SM, Jan G, Guyomarc’h F.
Langmuir. 2015 Jan.


> 2014

Optimized Protocol for Protein Macrocomplexes Stabilization Using the EDC, 1-Ethyl-3-(3-(dimethylamino)propyl)carbodiimide, Zero-Length Cross-Linker. 
Lepvrier E, Doigneaux C, Moullintraffort L, Nazabal A, Garnier C.
Anal Chem. 2014 Nov.

Overexpression of MLN51 triggers P-body disassembly and formation of a new type of RNA granules.
Cougot N, Daguenet E, Baguet A, Cavalier A, Thomas D, Bellaud P, Fautrel A, Godey F, Bertrand E, Tomasetto C, Gillet R.
J Cell Sci. 2014 Nov.

Bacterial trans-Translation: From Functions to Applications.
Giudice E, Gillet R.
In: "RNA Regulation, Current Topics from the Encyclopedia of Molecular Cell Biology and Molecular Medicine", (ed Robert A. Meyers). Wiley-VCH Verlag, Weinheim, Germany. 2014 Apr. pp 1-33

Becker muscular dystrophy severity is linked to the structure of dystrophin.
Nicolas A, Raguénès-Nicol C, Ben Yaou R, Ameziane-Le Hir S, Chéron A, Vié V, Claustres M, Leturcq F, Delalande O, Hubert JF, Tuffery-Giraud S, Giudice E, Le Rumeur E ; the French Network of Clinical Reference Centres for Neuromuscular Diseases (CORNEMUS).
Hum Mol Genet. 2014 Oct / 2015 Mar.

Trans-translation exposed: understanding the structures and functions of tmRNA-SmpB.
Giudice E, Macé K, Gillet R.
Front Microbiol. 2014 Mar.

Structure of glycoproteins from Acacia gum: an assembly of ring-like glycoproteins modules.
Renard D, Lepvrier E, Garnier C, Roblin P, Nigen M, Sanchez C.
Carbohydr Polym. 2014 Jan.

Visualizing compaction of polysomes in bacteria.
Cougot N, Molza AE, Delesques J, Giudice E, Cavalier A, Rolland JP, Ermel G, Blanco C, Thomas D, Gillet R.
J Mol Biol. 2014 Jan.


> 2013

MetAmyl: a METa-predictor for AMYLoid proteins.
Emily M, Talvas A, Delamarche C. 
PLoS One. 2013 Nov.

Molecular clues to the dystrophin-nNOS interaction: a theoretical approach.
Giudice E, Molza AE, Laurin Y, Nicolas A, Le Rumeur E, Delalande O.
Biochemistry. 2013 Nov.

The task force that rescues stalled ribosomes in bacteria. 
Giudice E, Gillet R.
Trends Biochem Sci. 2013 Aug.
>> Journal cover

Structural organization of the polysomes adjacent to mammalian processing bodies (P-bodies). 
Cougot N, Molza AE, Giudice E, Cavalier A, Thomas D, Gillet R.
RNA Biol. 2013 Feb.
>> Journal cover

An in vitro network of intermolecular interactions between viral RNA segments of an avian H5N2 influenza A virus: comparison with a human H3N2 virus.  
Gavazzi C, Isel C, Fournier E, Moules V, Cavalier A, Thomas D, Lina B, Marquet R.
Nucleic Acid Res. 2013 Jan.

Analyse in situ d’interactions macromoléculaires par Tomographie Electronique : l’exemple des « Processing Bodies ».
Cougot N, Giudice E, Gillet R, Thomas D.
Revue française d'Histotechnologie, Vol 26. Nancy, 2013.


> 2012

Interaction network linking the human H3N2 influenza A virus genomic RNA segments.
Fournier E, Moules V, Essere B, Paillart JC, Sirbat JD, Cavalier A, Rolland JP, Thomas D, Lina B, Isel C, Marquet R.
Vaccine. 2012 Dec

Origine, évolution et métamorphose du ribosome. À la recherche des vestiges d’un ancien "Monde ARN".
Gillet R.
In: "Variations, évolutions, métamorphoses", (eds: B. Pouderon &  J. Casas). Presses Universitaires de Saint-Etienne. 2012 Oct.

Structure of arabinogalactan-protein from Acacia gum: from porous ellipsoids to supramolecular architectures.
Renard D, Garnier C, Lapp A, Schmitt C, Sanchez C.
Carbohydr Polym. 2012 Sep.

The dual organization of P-bodies revealed by immunoelectron microscopy and electron tomography. 
Cougot N, Cavalier A, Thomas D, Gillet R.
J Mol Biol. 2012 Jun.
>> Journal cover

A Supramolecular Assembly Formed by Influenza A Virus Genomic RNA Segments.  
Fournier E, Moules V, Essere B, Paillart JC, Sirbat JD, Isel C, Cavalier A, Rolland JP, Thomas D, Lina B, Marquet R.
Nucleic Acids Res. 2012 Mar.

Decoding in the absence of a codon by tmRNA and SmpB in the ribosome. 
Neubauer C, Gillet R, Kelley AC, Ramakrishnan V.
Science. 2012 Mar.
>> This paper was highlighted in Nature Reviews Molecular Cell Biology 13, 280 (May 2012) and also featured in Faculty of 1000 Biology


> 2011

SmpB as the handyman of tmRNA during trans-translation.
Felden B, Gillet R.
RNA Biol. 2011 May-Jun.

Control of peptide nanotube diameter by chemical modifications of an aromatic residue involved in a single close contact.
Tarabout C, Roux S, Gobeaux F, Fay N, Pouget E, Meriadec C, Ligeti M, Thomas D, IJsselstijn M, Besselievre F, Buisson DA, Verbavatz JM, Petitjean M, Valéry C, Perrin L, Rousseau B, Franck Artzner F, Paternostre M, Cintrat JC.
Proc Natl Acad Sci U S A. 2011 May.

Importance of viral genomic composition in modulating glycoprotein content on the surface of influenza virus particles.
Moulès V, Terrier O, Yver M, Riteau B, Moriscot C, Ferraris O, Julien T, Giudice E, Roland JP, Erny A, Bouscambert-Duchamp M, Frobert E, Rosa-Calatrava M, Yi Pu L, Hay A, Thomas D, Schoehn G, Lina B. 
Virology. 2011 May.

Computational study of the human dystrophin repeats: interaction properties and molecular dynamics.
Legrand B, Giudice E, Nicolas A, Delalande O, Le Rumeur E.
PLoS One. 2011.


> 2010

tmRNA-SmpB: a journey to the centre of the bacterial ribosome.
Weis F, Bron P, Giudice E, Rolland JP, Thomas D, Felden B, Gillet R.
EMBO J. 2010 Nov.
>> This paper was highlighted in EMBO J (2010 Nov. 17;29(22):3747-9) and also featured in Faculty of 1000 Biology

In vitro characterization of naturally occurring influenza H3NA- viruses lacking the NA gene segment: toward a new mechanism of viral resistance?
Moules V, Ferraris O, Terrier O, Giudice E, Yver M, Rolland JP, Bouscambert Duchamp M, Bergeron C, Ottmann M, Fournier E, Traversier A, Boule C, Rivoire A, Yipu L, Hay A, Valette M, Marquet R, Rosa-Calatrava M, Naffakh N, Schoehn G, Thomas D, Lina B. 
Virology. 2010 Sep.

The molecular chaperone Hsp90's oligomers: when the bird makes its nest.
Moullintraffort L, Bruneaux M, Allegro D, Nazabal A, P. Barbier, Thomas D, Garnier C.
35th FEBS Congress "Molecules of Life, Gothenburg, Switzerland. 2010, Jun.

Biochemical and biophysical characterization of the Mg2+-induced 90-kDa heat shock protein oligomers.
Moullintraffort L, Bruneaux M, Nazabal A, Allegro D, Giudice E, Zal F, Peyrot V, Barbier P, Thomas D, Garnier  C. 
J Biol Chem. 2010 May.

The VLITL aggregation-prone motif might trigger amyloid fibril formation of fibrinogen A alpha-chain frameshift variants in vivo. 
Valleix S, Derreumaux P, Garnier C, Briki F, Boimard M, Doucet J, Rioux-Leclercq N, Martin L, Grateau G, Delpech M, Le Pogamp P.
XII International Symposium on Amyloidosis, "From Molecular Mechanisms Toward the Cure of Systemic Amyloidoses", Rome. 2010 Apr.

The 90-kDa heat shock protein HSP90 protects tubulin against thermal denaturation. 
Weis F, Moullintraffort L, Heichette C, Chrétien D, Garnier C.
J Biol Chem. 2010 Mar.

Elucidation of the self-assembly pathway of Lanreotide octapeptide into beta-sheet nanotubes: role of two stable intermediates.
Pouget E, Fay N, Dujardin E, Jamin N, Perrin L, Pandit A, Rose T, Valéry C, Thomas D, Paternostre M, Artzner F. 
J Am Chem Soc. 2010 Mar.

Accommodation of tmRNA-SmpB into stalled ribosomes: A cryo-EM study.
Weis F, Bron P, Rolland JP, Thomas D, Felden B, Gillet R. 
RNA. 2010 Feb.

Observation of membrane proteins in situ: AQPcic, the insect aquaporin example.
Thomas D, Cavalier A. 
In: "Membrane protein structure determination", (ed J.-J. Lacapere), in Series “Springer Protocols. Methods in Molecular Biology” (J. Walker, ser. ed.) Vol. 654. Humana Press, Springer, Heidelberg, 2010. pp 654:171-85


Articles publiés dans les comptes-rendus de congrès internationaux de bio-informatique

Comparison and Fusion Model in Protein Motifs.
Altamiranda J, Aguilar J, Delamarche C. 
Proceedings of the 2013 39th Latin American Computing Conference (CLEI), IEEE Xplore, Naiguata. 2013, Oct 7-11.

Algorithm based on the ant colony optimization for DNA motif fusion.
Altamiranda J, Aguilar J, Torres R, Delamarche C. 
Mathematical Models and Methods in Modern Science, Porto. 2012.

Similarity of Amyloid Protein Motif using an Hybrid Intelligent System.
[Similitud de Motivos de Proteínas Amiloideas utilizando un Sistema Hibrido Inteligente]
[Comparacion y fusion de Motivos de Proteina β-amiloidea utilizando Computacion Inteligente]

Altamiranda J, Aguilar J, Delamarche C.
Latin America Transactions [Revista IEEE America Latina] IEEE Xplore. 2011, Sept 29.