Publications de l'équipe Dynamique et Mécanique des Epithélia


ESCRT-III-dependent adhesive and mechanical hanges are triggered by a mechanism detceting alteration of septate junction integrity in Drosophila epithelial cells   Thomas Esmangart de Bournonville, Mariusz Jaglarz, Emeline Durel and Roland Le Borgne  eLife (2024)

Assembly, Dynamics and remodeling of epithelial cell junctions throughout development  Marta Mira Osuna and Roland Le Borgne  Development (2024)  10.1242/dev.201086


Cell-intrinsic and -extrinsic roles of the ESCRT-III subunit Shrub in abscission of Drosophila sensory organ precursor  Céline Bruelle, Mathieu Pinot, Emeline Daniel, Marion Daudé, Juliette Mathieu and Roland Le Borgne Development (2023) doi: 10.1242/dev.201409


Bazooka/Par3 cooperates with Sanpodo for the assembly of Notch signaling clusters following asymmetric division of Drosophila sensory organ precursor cells  Elise Houssin, Mathieu Pinot, Karen  Bellec, and Roland Le Borgne eLife (2021)doi:,

Clathrin adaptor AP-1 and Stratum act in parallel pathways to control Notch activation in Drosophila Sensory Organ Precursor Cells K. Bellec, M. Pinot, I. Gicquel and R. Le Borgne Development. 2021 Jan 10;148(1):dev191437. DOI: 10.1242/dev.191437

Super-resolved live-cell imaging using Random Illumination Microscopy
Thomas Mangeat, Simon Labouesse, Marc Allain, Awoke Negash, Emmanuel Martin, Aude Guénolé, Renaud Poincloux, Claire Estibal, Anaïs Bouissou, Sylvain Cantaloube, Elodie Vega, Tong Li, Christian Rouvière, Sophie Allard, Debora Keller, Valentin Debarnot, Xia Bo Wang,  Grégoire Michaux, Mathieu Pinot, Roland Le Borgne, Sylvie Tournier, Magali Suzanne, Jérôme Idier, Anne Sentenac.
2021, Cell Reports Methods

Mitochondrial Aurora kinase A induces mitophagy by interacting with MAP1LC3 and Prohibitin2 Giulia Bertolin, Marie-Clotilde Alves-Guerra, Agnès Burel, Claude Prigent, V Roland Le Borgne, Marc Tramier (2020) BioRXiv,  doi: (2020), accepted in Life Science Alliance (EMBO Press)


Interplay between Anakonda, Gliotactin, and M6 for Tricellular Junction Assembly and Anchoring of Septate Junctions in Drosophila Epithelium. Thomas Esmangart de Bournonville  1 Roland Le Borgne  2  Current Biology  2020 Nov 2;30(21):4245-4253.e4. DOI: 10.1016/j.cub.2020.07.090

Super-resolved live-cell imaging using Random Illumination Microscopy T. Mangeat, S. Labouesse, M.Allain, E. Martin, R. Poincloux, A. Bouissou, S. Cantaloube, E. Courtaux, E. Vega, T. Li, A. Guénolé, C. Rouvière, S. Allard, N. Campo, M. Suzanne, X.  Wang, G. Michaux, M. Pinot, R. Le Borgne, S. Tournier, J. Idier, A. Sentenac  BioRXiv 2020


Force Transmission between Three Tissues Controls Bipolar Planar Polarity Establishment and Morphogenesis Gillard G, Nicolle O, Brugière T, Prigent S, Pinot M, Michaux G.  2019, Current Biology


Stratum recruits Rab8 at Golgi exit sites to regulate the basolateral sorting of Notch and Sanpodo Bellec, K., Gicquel, I., Le Borgne R. Development 2018 Jul2; 154(13) pii: dev163469. DOI: 10.1242/dev.163469

Coordination of Septate Junctions Assembly and Completion of Cytokinesis in Proliferative Epithelial Tissues Daniel, E., Daudé, M., Kolotuev I., Charish, K., Auld V., Le Borgne R.  Curr Biol. 2018 May 7;28(9):1380-1391.e4. doi: 10.1016/j.cub.2018.03.034. Epub 2018 Apr 26. DOI: 10.1016/j.cub.2018.03.034

Centrosomal ALIX regulates mitotic spindle orientation by modulating astral microtubule dynamics L. Malerod*, R. Le Borgne*, A. Lie-Jensen, Å.H. Eikenes, A. Brech, K. Liestøl, H.Stenmark and K.Haglund  EMBO J. 2018 Jul 2;37(13). pii: e97741. (co-first author) DOI: 10.15252/embj.201797741

AURKA controls mitochondrial fusion and mitophagy to increase energy production in cancer cells.  G. Bertolin, A-L. Bulteau, M-C. Alves-Guerra, A. Burel, M-T. Lavault, O. Gavard, S. Le Bra, J-P. Gagné, G. G. Poirier, O. Delalande, R. Le Borgne, C. Prigent  and M.Tramier (2018)  eLife, Aug 2;7. pii: e38111. DOI: 10.7554/eLife.38111


Remodeling of adhesion and modulation of mechanical tensile forces during apoptosis in Drosophila epithelium X. Teng, L. Qin, R. Le Borgne and Y. Toyama Development 2017 144(1): 95-105 DOI: 10.1242/dev.139865


Drosophila E-cadherin is required for the maintenance of ring canals anchoring to mechanically withstand tissue growth
Loyer N., Kolotuev I., Pinot M., Le Borgne R.

Proc Natl Acad Sci U S A. 2015 Oct 13;112(41):12717-22.

Epithelial cell division – multiplying without losing touch.
Le Bras S and Le Borgne R.
J. Cell Science. 2014 Dec.

Numb inhibits the recycling of Sanpodo in Drosophila sensory organ precursor. 
Cotton M, Benhra N, and Le Borgne R.

Curr Biology. 2013 Apr.
>> Comment in Current Biology 23 (7) R270-2

Septins regulate the contractility of the actomyosin ring to enable adherens junction remodeling during cytokinesis of epithelial cells. 
Founounou N, Loyer N and Le Borgne R.

Dev Cell. 2013 Feb.
>> Cited in Faculty of 1000 Biology & Comment in Developmental Cell 24(4) 336-8 

Spatiotemporal control of microtubule nucleation and assembly using magnetic nanoparticles.
Hoffmann C,  Mazari E, Lallet S, Le Borgne R, Marchi-Artzner V, Gosse C and Gueroui Z.  

Nat Nanotechnology. 2013 Mar.

Drosophila S2 Cells Secrete Wingless on Exosome-Like Vesicles but the Wingless Gradient Forms Independently of Exosomes. 
Beckett K, Monier S, Palmer L, Alexandre C, Green H, Bonneil E, Raposo G, Thibault P, Le Borgne R, Vincent JP. 

Traffic. 2013 Jan.

Genetic identification of intracellular trafficking regulators involved in Notch-dependent binary cell fate acquisition following asymmetric cell division.
Le Bras S, Rondanino C, Kriegel-Taki G, Dussert A and Le Borgne R. 

J. Cell Science. 2012 Oct.

Tissue polarity: PCP inheritance ensured by selective mitotic endocytosis. 
Founounou N. and Le Borgne R. 

Curr Biology. 2011 Sep.
>> Dispatch

AP-1 and clathrin are essential for secretory granule biogenesis in Drosophila. 
Burgess J, Jauregui M, Tan J, Rollins J, Lallet S, Leventis PA, Boulianne GL, Chang HC, Le Borgne R, Krämer H, Brill JA. 
Mol Bio Cell. 2011 Jun.

The multiple facets of ubiquitination in the regulation of Notch signaling pathway. 
Le Bras S, Loyer N and Le Borgne R.
Traffic. 2011 Feb.
>> Review

AP-1 controls the trafficking of Notch and Sanpodo toward E-Cadherin junctions in sensory organ precursors. 
Benhra N, Lallet L, Cotton M, Le Bras S, Dussert A and Le Borgne R. 

Curr Biology. 2011 Jan
>> Cited in Faculty of 1000 Biology

Neuralized Promotes Basal to Apical Transcytosis of Delta in Epithelial Cells. 
Benhra N, Vignaux F, Dussert A, Schweisguth F and Le Borgne R. 

Mol Bio Cell. 2010 Jun.
>> Cited in Faculty of 1000 Biology

Avant 2010

Small and stable peptidic PEGylated quantum dots to target polyhistidine-tagged proteins with controlled stoichiometry.
Dif A, Boulmedais F, Pinot M, Roullier V, Baudy-Floc'h M, Coquelle FM, Clarke S, Neveu P, Vignaux F, Le Borgne R, Dahan M, Gueroui Z, Marchi-Artzner V.

J Am Chem Soc. 2009 Oct.

Tri sélectif et recyclage : Wntless et le retromer, deux acteurs clés de la signalisation WNT [Sorting, recycling and WNT signaling: Wntless and retromer functions]. 
Michaux G, Le Borgne R. 
Med Sci. 2009 Jun-Jul.
>> Review

aPKC-mediated phosphorylation regulates asymmetric membrane localization of the cell fate determinant Numb. 
Smith CA, Lau KM, Rahmani Z, Dho SE, Brothers G, She YM, Berry DM, Bonneil E, Thibault P, Schweisguth F, Le Borgne R, McGlade CJ. 

EMBO J. 2007 Jan.
>> Cited in Faculty of 1000 Biology

Regulation of Notch signaling by endocytosis and endosomal sorting. 
Le Borgne R. 
Curr Opin Cell Biol. 2006 Apr.
>> Review

Lethal giant larvae controls the localisation of the Notch signaling regulators Numb, Neuralized and Sanpodo in Drosophila sensory organ precursor cells. 
Langevin J, Le Borgne R*, Rosenfeld F, Gho M, Schweisguth F and Bellaïche Y. 

Curr Biology. 2005 May. - * Equal contribution
>> Cited in Faculty of 1000 Biology

The roles of receptor and ligand endocytosis in the regulation of Notch signaling. 
Le Borgne R, Bardin A and Schweisguth F. 

Development. 2005 Apr.
>> Review

Two distinct E3 ubiquitin ligases have complementary functions in the regulation of delta and serrate signaling in Drosophila.
Le Borgne R, Remaud S, Hamel S, Schweisguth F. 

PLoS Biology. 2005 Apr.
>> Cited in Faculty of 1000 Biology

Asymmetric localization and function of cell-fate determinants: a fly's view. 
Bardin AJ, Le Borgne R, Schweisguth F. 

Curr Opin Neurobiol. 2004 Feb.
>> Review

Unequal segregation of Neuralized biases Notch activation during asymmetric cell division. 
Le Borgne R and Schweisguth F. 

Dev Cell. 2003 Jul.
>> Cited in Faculty of 1000 Biology

Notch signaling: endocytosis makes Delta signal better. 
Le Borgne R and Schweisguth F. 

Curr Biology. 2003 Apr.
>> Dispatch  

Snail is required for Delta endocytosis and Notch-dependent activation of single-minded expression in Drosophila.
Morel V, Le Borgne R and Scweisguth F.

Dev Genes Evol. 2003 Mar.

Visualization of TGN to Endosome Trafficking through Fluorescently Labeled MPR and AP-1 in Living Cells. 
Waguri S, Dewitte F, Le Borgne R, Rouille Y, Uchiyama Y, Dubremetz JF and Hoflack B. 

Mol Biol Cell. 2003 Jan.
>> Cited in Faculty of 1000 Biology

Drosophila E-Cadherin regulates the orientation of asymetric cell division in the sensory organ lineage.
Le Borgne R, Bellaïche Y and Schweisguth F.

Curr Biology. 2002 Jan.

The AP-3 dependent targeting of the melanosomal glycoprotein QNR71 requires its di-leucine-based sorting signal. 
Le Borgne R, Martin P, Planque N, Dewitte F, Saule S and Hoflack B. 

J Cell Sci. 2001 Aug.

Wasp, The Drosophila Wiskott-Aldrich Syndrome Gene Homolog, Is Required For Cell Fate Decisions Mediated By Notch Signaling. 
Ben-Yaacov S, Le Borgne R, Abramson I, Schweisguth F and Schejter ED. 

J Cell Biol. 2001 Jan.

The mammalian AP-3 adaptor-like complex mediates the intracellular transport of lysosomal membrane glycoproteins.  
Le Borgne R, Alconada A, Bauer U, and Hoflack B. 

J Biol Chem. 1998 Nov.

Mechanisms of protein sorting and coat assembly: insights from the clathrin-coated vesicle pathway. 
Le Borgne R and Hoflack B. 

Curr Opin Cell Biol. 1998 Aug.
>> Review

Protein transport from the secretory to the endocytic pathway in mammalian cells. 
Le Borgne R and Hoflack B. 

Bioch Biophys Acta. 1998 Aug.
>> Review

Basolateral sorting of the cation-dependent mannose 6-phosphate receptor in Madin-Darby Canine Kidney cells: identification of a basolateral determinant unrelated to clathrin-coated pit localization signals. 
Distel B, Bauer U, Le Borgne R and Hoflack B. 

J Biol Chem. 1998 Jan.

Mannose 6-Phosphate Receptors Regulate the Formation of Clathrin-coated Vesicles in the TGN
Le Borgne R and Hoflack B. 

J Cell Biol. 1997 Apr.

Expression of major histocompatibility complex class II molecules in HeLa cells promotes the recruitment of AP-1 Golgi-specific assembly proteins on Golgi membranes. 
Salamero J, Le Borgne R, Saudrais C, Goud B and Hoflack B. 

J Biol Chem. 1996 Nov.

A casein kinase II phosphorylation site in the cytoplasmic domain of the cation-dependent mannose 6-phosphate receptor determines the high affinity interaction of the AP-1 Golgi-assembly proteins with membranes. 
Mauxion F, Le Borgne R, Munier-Lehmann H and Hoflack B.

J Biol Chem. 1996 Jan.

Mannose 6-phosphate receptors and ADP-ribosylation factors cooperate for high affinity interaction of the AP-1 Golgi-assembly proteins with membranes. 
Le Borgne R, Griffiths G and Hoflack B. 

J Biol Chem. 1996 Jan.

Roles for mannose 6-phosphate receptors in lysosomal emzyme sorting, IGF-II binding and clathrin-coat assembly. 
Ludwig T, Le Borgne R and Hoflack B. 

Trends Cell Biol. 1995 May.
>> Review

Chimeric proteins containing the cytoplasmic domains of the mannose 6-phosphate receptors codistributes with the endogenous receptors. 
Mauxion F, Schmidt A, Le Borgne R and Hoflack B. 

Eur J Cell Biol. 1995 Feb.

Ezrin has properties to self-associate at the plasma membrane. 
Andréoli C, Martin M, Le Borgne R, Reggio H and Mangeat P. 
J. Cell Science. 1994 Sep.

Binding of AP-1 Golgi adaptors to membranes requires phosphorylated cytoplasmic domains of the mannose 6-phosphate/insulin-like growth factor II receptor. 
Le Borgne R, Schmidt A, Mauxion F, Griffiths G and Hoflack B. 

J. Biol. Chem. 1993 Oct.

Effects of discrete lesions in the ventral noradrenergic ascending bundle on the corticotropic stress response depend on the site of the lesion and on the plasma levels of adrenal steroids. 
Gaillet S, Alonso G, Le Borgne R, Barbanel G, Malaval F, Assenmacher I, Szafarczyk A.

Neuroendocrinology. 1993 Oct.