> 2022

Pivotal role of PIM2 kinase in plasmablast generation and plasma cell survival, opening new treatment options in myeloma.
Haas M, Caron G, Chatonnet F, Manenti S, Alaterre E, Devin J, Delaloy C, Bertolin G, Viel R, Pignarre A, et al.
Blood blood.2021014011.

A comparison of high resolution image reconstruction methods from confocal array detector with a new deconvolution algorithm.
Prigent S, Dutertre S, Bidaud-Meynard A, Bertolin G, Michaux G, Kervrann C.

Mitochondrial transport, partitioning, and quality control at the heart of cell proliferation and fate acquisition.
Kumar Sharma R, Chafik A, Bertolin G.
American Journal of Physiology-Cell Physiology (2022); 322:C311–25.

Neural network fast-classifies biological images through features selecting to power automated microscopy.
Balluet M, Sizaire F, El Habouz Y, Walter T, Pont J, Giroux B, Bouchareb O, Tramier M, Pecreaux J.
J Microsc. (2022) 285:3-19.


> 2021

Engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imaging.
Benaissa H, Ounoughi K, Aujard I, Fischer E, Goïame R, Nguyen J, Tebo AG, Li C, Le Saux T, Bertolin G, Tramier M, Danglot L, Pietrancosta N, Morin X, Jullien L, Gautier A.
Nat Commun (2021) 12:6989.

tdLanYFP, a Yellow, Bright, Photostable, and pH-Insensitive Fluorescent Protein for Live-Cell Imaging and Förster Resonance Energy Transfer-Based Sensing Strategies.
Bousmah Y, Valenta H, Bertolin G, Singh U, Nicolas V, Pasquier H, Tramier M, Merola F, Erard M.
ACS Sens. (2021) 6:3940-3947.

Label-free microscopy of mitotic chromosomes using the polarization orthogonality breaking technique. Biomed Opt Express.
Desapogu R, Le Marchand G, Smith R, Ray P, Gillier E, Dutertre S, Alouini M, Tramier M, Huet S, Fade J.
Biomed Opt Express. (2021) 12:5290-5304.

Quantitative dSTORM super-resolution microscopy localizes Aurora kinase A/AURKA in the mitochondrial matrix.
Durel B, Kervrann C, Bertolin G.
Biology of the Cell (2021); 113:458–73.

Approaches to monitor ATP levels in living cells: where do we stand?
Ley-Ngardigal S, Bertolin G.
The FEBS Journal (2021)

Mitochondrial Aurora kinase A induces mitophagy by interacting with MAP1LC3 and Prohibitin 2.
Bertolin G, Alves-Guerra MC, Cheron A, Burel A, Prigent C, Le Borgne R, Tramier M.
LSA. (2021) 4:e202000806.

The NANOTUMOUR consortium – Towards the Tumor Cell Atlas.
Colin F, Schauer K, Hamiche A, Martineau P, Borg JP, Bednar J, Bertolin G, Camoin L, Collette Y, Dimitrov S, Fournier I, Hyenne V, Mendoza-Parra MA, Morelli X, Rondé P, Sumara I, Tramier M, Schultz P, Goetz JG.
Biol Cell. (2021) 113:272-280.

3D quantification of autophagy activation and autophagosome-to-mitochondria recruitment in a Drosophila model of Parkinson’s disease.
Gutierrez-Luke SJ, Juba AN, Bertolin G, Buhlman LM.
STAR Protocols (2021); 2:100408.

> 2020

Real-Time Monitoring of Aurora kinase A Activation using Conformational FRET Biosensors in Live Cells. J Vis Exp.
Bertolin G, Le Marchand G, Tramier M.
J Vis Exp. (2020) 161. doi: 10.3791/61611.

Hepatitis B Virus Core Protein Domains Essential for Viral Capsid Assembly in a Cellular Context.
Rat V, Pinson X, Seigneuret F, Durand S, Herrscher C, Lemoine R, Burlaud-Gaillard J, Raynal PY, Hourioux C, Roingeard P, Tramier M, de Rocquigny H.
J Mol Biol. (2020) 432:3802-3819.

Automated screening of AURKA activity based on a genetically encoded FRET biosensor using Fluorescence Lifetime Imaging Microscopy.
Sizaire F, Le Marchand G, Pécréaux J, Bouchareb O, Tramier M.
Methods Appl Fluoresc. (2020) 8:024006.

Imaging Mitochondrial Functions: from Fluorescent Dyes to Genetically-Encoded Sensors.
Gökerküçük EB, Tramier M, Bertolin G.
Genes (2020) 11. pii: E125.

Insights into the non-mitotic functions of Aurora kinase A: more than just cell division
Bertolin G, Tramier M.
Cell. Mol. Life Sci. (2019) doi:10.1007/s00018-019-03310-2

> 2019

Optimized FRET pairs and quantification approaches to detect the activation of Aurora kinase A at mitosis.
Bertolin G, Sizaire F, Demeautis C, Chapuis C, Merola F, Erard M, Tramier M.
ACS Sens. (2019) 4:2018-2027.

Chromatin condensation fluctuations rather than steady-state predict chromatin accessibility.
Audugé N, Padilla-Parra S, Tramier M, Borghi N, Coppey-Moisan M.
Nucleic Acids Res. (2019) 47:6184-6194.

Quantitative live-cell imaging and 3D modeling reveal critical functional features in the cytosolic complex of phagocyte NADPH oxidase.
Ziegler CS, Bouchab L, Tramier M, Durand D, Fieschi F, Dupré-Crochet S, Mérola F, Nüße O, Erard M.
J Biol Chem. (2019) 294:3824-3836.

> 2018

The polarity-induced force imbalance in Caenorhabditis elegans embryos is caused by asymmetric binding rates of dynein to the cortex.
Rodriguez-Garcia R, Chesneau L, Pastezeur S, Roul J, Tramier M, Pécréaux J.
Mol Biol Cell. (2018) mbcE17110653.

Aurora kinase A localises to mitochondria to control organelle dynamics and energy production.
Bertolin G, Bulteau AL, Alves-Guerra MC, Burel A, Lavault MT, Gavard O, Le Bras S, Gagné JP, Poirier GG, Le Borgne R, Prigent C, Tramier M.
Elife. (2018) 7. pii: e38111. 

> 2017

Bioconjugated fluorescent organic nanoparticles targeting EGFR-overexpressing cancer cells.
Faucon A, Benhelli-Mokrani H, Fleury F, Dutertre S, Tramier M, Boucard J, Lartigue L, Nedellec S, Hulin P, Ishow E.
Nanoscale. (2017) 9:18094–18106.

Multiplexing molecular tension sensors reveals piconewton force gradient across talin-1.
Ringer P, Weißl A, Cost AL, Freikamp A, Sabass B, Mehlich A, Tramier M, Rief M, Grashoff C.
Nat Methods. (2017) 14:1090-1096.​

Actomyosin-generated tension on cadherin is similar between dividing and non-dividing epithelial cells in early​.
Herbomel G, Hatte G, Roul J, Padilla-Parra S, Tassan JP, Tramier M.
Sci Rep. (2017) 7:45058.

Multiplexing PKA and ERK1&2 kinases FRET biosensors in living cells using single excitation wavelength dual colour FLIM.
Demeautis C, Sipieter F, Roul J, Chapuis C, Padilla-Parra S, Riquet FB, Tramier M.
Sci Rep. (2017) 7:41026.

> 2016

A FRET biosensor reveals spatiotemporal activation and functions of Aurora kinase A in living cells.
Bertolin G, Sizaire F, Herbomel G, Reboutier D, Prigent C, Tramier M.
Nat Commun. (2016) 7:12674.

> 2015

A high-throughput direct fluorescence resonance energy transfer-based assay for analyzing apoptotic proteases using flow cytometry and fluorescence lifetime measurements.
Suzuki M, Sakata I, Sakai T, Tomioka H, Nishigaki K, Tramier M, Coppey-Moisan M.
Anal Biochem. 2015 Dec.  

Time-domain fluorescence lifetime imaging microscopy: a quantitative method to follow transient protein-protein interactions in living cells.
Padilla-Parra S, Audugé N, Tramier M, Coppey-Moisan M.
Cold Spring Harb Protoc. 2015 Jun.

> 2014

Golgi sorting regulates organization and activity of GPI proteins at apical membranes. 
Paladino S, Lebreton S, Tivodar S, Formiggini F, Ossato G, Gratton E, Tramier M, Coppey-Moisan M, Zurzolo C.
Nat Chem Biol. 2014 May.

Epithelial cell division in the Xenopus laevis embryo during gastrulation.
Hatte G, Tramier M, Prigent C, Tassan JP.
Int J Dev Biol. 2014.

Quantitative study of protein-protein interactions in live cell by dual-color fluorescence correlation spectroscopy. 
Padilla-Parra S, Audugé N, Coppey-Moisan M, Tramier M.
Methods Mol Biol. 2014.

> 2013

Spatio-temporal quantification of FRET in living cells by fast Time-Domain FLIM: a comparative study of non-fitting methods.
Leray A, Padilla-Parra S, Roul J, Héliot L, Tramier M.
PLoS One. 2013 Jul.

> 2012

FRET microscopy in the living cell: Different approaches, strengths and weaknesses. 
Padilla-Parra S, Tramier M.
BioEssays. 2012 May.

Live-cell imaging reveals multiple interactions between Epstein-Barr Nuclear Antigen 1 (EBNA-1) and cellular chromatin during interphase and mitosis.
Jourdan N, Jobart-Malfait A, Dos Reis G, Quignon F, Piolot T, Klein C, Tramier M, Coppey M, Marechal V.
J Virol. 2012 May.

> 2011

Dual-color fluorescence lifetime correlation spectroscopy to quantify protein-protein interactions in live cell.
Padilla-Parra S, Audugé N, Coppey-Moisan M, Tramier M.
Microsc Res Tech. 2011 Aug.

Non fitting based FRET–FLIM analysis approaches applied to quantify protein–protein interactions in live cells.
Padilla-Parra S, Audugé N, Coppey-Moisan M, Tramier M.
Biophys Rev. 2011 Jun.

Time-Domain Fluorescence Lifetime Imaging Microscopy: A Quantitative Method to Follow Transient Protein–Protein Interactions in Living Cells.
Padilla-Parra S, Audugé N, Tramier M, Coppey-Moisan M.
In "Imaging: A Laboratory Manual" (ed. Yuste). CSHL Press, Cold Spring Harbor, NY, USA. 2011.

Before 2010

> 2009

Dynamic interaction of amphiphysin with N-WASP regulates actin assembly.
Yamada H, Padilla-Parra S, Park SJ, Itoh T, Chaineau M, Monaldi I, Cremona O, Benfenati F, De Camilli P, Coppey-Moisan M, Tramier M, Galli T, Takei K.
J Biol Chem. 2009 Dec.

Quantitative comparison of different fluorescent protein couples for fast FRET-FLIM acquisition.
Padilla-Parra S, Audugé N, Lalucque H, Mevel JC, Coppey-Moisan M, Tramier M.
Biophys J. 2009 Oct.

Spatio-temporal analysis of cell response to a rigidity gradient: a quantitative study by multiple optical tweezers.
Allioux-Guerin M, Icard-Arcizet D, Durieux C, Henon S, Gallet F, Mevel JC, Masse MJ, Tramier M, Coppey-Moisan M.
Biophys J. 2009 Jan.

> 2008

Time-lapse Microscopy and Fluorescence Resonance Energy Transfer to Analyze the Dynamics and Interactions of Nucleolar Proteins in Living Cells.
Louvet E, Tramier M, Angelier N, Hernandez-Verdun D.
Methods Mol Biol. 2008.

Quantitative FRET analysis by fast acquisition time domain FLIM at high spatial resolution in living cells.
Padilla-Parra S, Audugé N, Coppey-Moisan M, Tramier M.
Biophys J. 2008 Sep.

Fluorescence anisotropy imaging microscopy for homo-FRET in living cells.
Tramier M, Coppey-Moisan M.
Methods Cell Biol. 2008.

> 2007

beta-arrestin 2 oligomerization controls the Mdm2-dependent inhibition of p53.
Boularan C, Scott MG, Bourougaa K, Bellal M, Esteve E, Thuret A, Benmerah A, Tramier M, Coppey-Moisan M, Labbé-Jullié C, Fåhraeus R, Marullo S.
Proc Natl Acad Sci U S A. 2007 Nov

> 2006

Sensitivity of CFP/YFP and GFP/mCherry pairs to donor photobleaching on FRET determination by fluorescence lifetime imaging microscopy in living cells.
Tramier M, Zahid M, Mevel JC, Masse MJ, Coppey-Moisan M.
Microsc Res Tech. 2006 Nov.

The truncated prelamin A in Hutchinson-Gilford progeria syndrome alters segregation of A-type and B-type lamin homopolymers.
Delbarre E, Tramier M, Coppey-Moisan M, Gaillard C, Courvalin JC, Buendia B.
Hum Mol Genet. 2006 Apr.

> 2005

Homo- and hetero-oligomerization of beta-arrestins in living cells.
Storez H, Scott MG, Issafras H, Burtey A, Benmerah A, Muntaner O, Piolot T, Tramier M, Coppey-Moisan M, Bouvier M, Labbé-Jullié C, Marullo S.
J Biol Chem. 2005 Dec.

Tracking the interactions of rRNA processing proteins during nucleolar assembly in living cells.
Angelier N, Tramier M, Louvet E, Coppey-Moisan M, Savino TM, De Mey JR, Hernandez-Verdun D.
Mol Biol Cell. 2005 Jun.

Encapsulation of magnetic and fluorescent nanoparticles in emulsion droplets.
Mandal SK, Lequeux N, Rotenberg B, Tramier M, Fattaccioli J, Bibette J, Dubertret B.
Langmuir. 2005 Apr.

> 2004

Picosecond time-resolved microspectrofluorometry in live cells exemplified by complex fluorescence dynamics of popular probes ethidium and cyan fluorescent protein.
Tramier M, Kemnitz K, Durieux C, Coppey-Moisan M.
J Microsc. 2004 Feb.

FRET and Fluorescence Lifetime Imaging Microscopy.
M. Tramier, D. Sanvitto, V. Emiliani, C. Durieux, and M. Coppey-Moisan.
In "CSHL : a laboratory manual". CSHL Press, Cold Spring Harbor, NY, USA. 2004.

Homo-FRET versus hetero-FRET to probe homodimers in living cells.
Tramier M, Piolot T, Gautier I, Mignotte V, Coppey J, Kemnitz K, Durieux C, Coppey-Moisan M.
Methods Enzymol. 2003

Low intensity 2D imaging of fluorescence lifetimes in living cells.
Emiliani V, Sanvitto D, Tramier M, Piolot T, Pettrasek Z, Kemnitz K, Durieux C, Coppey-Moisan M.
Appl Phys Lett. 2003. 

> 2002

Picosecond-hetero-FRET microscopy to probe protein-protein interactions in live cells.
Tramier M, Gautier I, Piolot T, Ravalet S, Kemnitz K, Coppey J, Durieux C, Mignotte V, Coppey-Moisan M.
Biophys J. 2002 Dec.

> 2001

Homo-FRET microscopy in living cells to measure monomer-dimer transition of GFP-tagged proteins.
Gautier I, Tramier M, Durieux C, Coppey J, Pansu RB, Nicolas JC, Kemnitz K, Coppey-Moisan M.
Biophys J. 2001 Jun.

Close but distinct regions of human herpesvirus 8 latency-associated nuclear antigen 1 are responsible for nuclear targeting and binding to human mitotic chromosomes.
Piolot T, Tramier M, Coppey M, Nicolas JC, Marechal V.
J Virol. 2001 Apr.

Binding of Ethidium to Yeast tRNAPhe : A New Perspective on an Old Bromide.
M. Tramier, O. Holub, J. C. Croney, T. Ishii, S. E. Seifried, and D. M. Jameson.
MAFS7 Book Meeting. 2001.

> 2000

Restrained torsional dynamics of nuclear DNA in living proliferative mammalian cells.
Tramier M, Kemnitz K, Durieux C, Coppey J, Denjean P, Pansu RB, Coppey-Moisan M.
Biophys J. 2000 May.