Responsable de l'équipe

Email : roland [dot] Leborgne [at] univ-rennes [dot] fr

Phone : +33 (0)2 23 23 48 94

roland [dot] Leborgneatuniv-rennes1 [dot] fr (Write a message)

In a few words

After my PhD in Cell Biology with B. Hoflack at EMBL (European Molecular Biology Laboratory, Heidelberg, Germany), during which I was interested in membrane trafficking, I joined the lab of F. Schweisguth at the Ecole Normale Supérieure (Paris) to combine cell biology and developmental biology approaches. I have been studying asymmetric cell division and Notch dependent binary cell fate acquisition using Drosophila as a model organism. There, I first show that cell-cell contact provide a positional cue to orient asymmetric cell division. Second, I showed that the E3 ubiquitin ligase Neuralized segregates unequally during asymmetric cell division, to control the endocytic trafficking of the Notch ligand, a process essential for binary activation of Notch. I also showed that a second E3 ubiquitin ligase called Mind Bomb has a complementary function to Neuralized during Drosophila development, leading to the concept that Notch activation always requires the endocytosis of its ligands. Since my arrival at the IGDR, my lab is interested in the molecular mechanisms of epithelial cell division, linking the transmission of epithelial barrier functions to the spatio-temporal activation of the Notch pathway during cytokinesis. 

Key Articles

1- Thomas Esmangart de Bournonville, Roland Le Borgne (2020) Interplay between Anakonda, Gliotactin and M6 for tricellular junction assembly and anchoring of septate junctions in Drosophila epithelium Curr Biol 2020 Nov 2;30(21):4245-4253.e4. ; doi: 10.1016/j.cub.2020.07.090. Epub 2020 Aug 27.

2- E. Daniel, I. Kolotueva, M. Daudé, K. Charish, V. Auld and R. Le Borgne (2018) Coordination of septate junctions assembly and completion of cytokinesis in proliferative epithelial tissues, Curr Biol. 2018 May 7;28(9):1380-1391.e4. doi: 10.1016/j.cub.2018.03.034. Epub 2018 Apr 26.

3- N. Loyer, I. Kolotuev, M. Pinot, R. Le Borgne. ‘Drosophila E-cadherin is required for the maintenance of ring canals anchoring to mechanically withstand tissue growth. Proc Natl Acad Sci U S A. 2015 Oct 13;112(41):12717-22.

4- Founounou, N., Loyer, N. and Le Borgne, R. (2013) Septins regulate the contractility of the actomyosin ring to enable adherens junction remodeling during cytokinesis of epithelial cells. (2013) Developmental cell 24 (3), pp242-255. Cited in Faculty of 1000 Biology & comment in Developmental  Cell 24(4) 336-8

5- Benhra, N., Lallet, L., Cotton, M., Le Bras, S., Dussert, A., and Le Borgne, R. (2011). AP-1 controls the trafficking of Notch and Sanpodo toward E-Cadherin junctions in sensory organ precursors. Current  Biology 21 (1), 87-95. Cited in Faculty of 1000 Biology

6- Le Borgne, R., Remaud, S., Hamel,S., and Schweisguth F (2005). The E3 ubiquitin ligases Mind bomb and Neuralized have distinct and complementary functions in the regulation of Delta and Serrate activity in drosophila. PLoS Biology 3, e96. Cited in Faculty of 1000 Biology

7- Le Borgne, R. and Schweisguth, F. (2003). Unequal segregation of Neuralized biases Notch activation during asymmetric cell division. Developmental  Cell  5(1), 139-148. Cited in Faculty of 1000 Biology

8- Le Borgne R., Bellaïche Y.and Schweisguth F.(2002) Drosophila E-Cadherin regulates the orientation of asymetric cell division in the sensory organ lineage, Current Biology, 12, 95-104

9- Le Borgne,R., and Hoflack,B. (1997). The mannose 6-phosphate receptors regulate the formation of clathrin-coated vesicles in the trans-Golgi network. J. Cell Biol. 137: 335-345

10- Le Borgne, R., Schmidt,A., Mauxion,F., Griffiths,G., and Hoflack,B. (1993). Binding of AP-1 Golgi adaptors to membranes requires phosphorylated cytoplasmic domains of the mannose 6-phosphate/insulin-like growth factor II receptor. J. Biol. Chem. 268: 22552-22556

11- Andréoli,C., Martin,M., Le Borgne,R., Reggio,H., and Mangeat,P. (1994). Ezrin has properties to self-associate at the plasma membrane. J. Cell Science 107: 2509-2521