The team aims at developing techniques and methodologies in fluorescence microscopy to study dynamics of protein-protein interactions and biochemical activities in live sample. Team approaches are mainly driven by methodological and technological development, its transfer and its applicability in biology to answer relevant new questions.


Aurora kinase A localises to mitochondria to control organelle dynamics and energy production.
Bertolin G, Bulteau AL, Alves-Guerra MC, Burel A, Lavault MT, Gavard O, Le Bras S, Gagné JP, Poirier GG, Le Borgne R, Prigent C, Tramier M.
Elife. (2018) 7. pii: e38111. 

Multiplexing molecular tension sensors reveals piconewton force gradient across talin-1.
Ringer P, Weißl A, Cost AL, Freikamp A, Sabass B, Mehlich A, Tramier M, Rief M, Grashoff C.
Nat Methods. (2017) 14:1090-1096.​

Multiplexing PKA and ERK1&2 kinases FRET biosensors in living cells using single excitation wavelength dual colour FLIM.
Demeautis C, Sipieter F, Roul J, Chapuis C, Padilla-Parra S, Riquet FB, Tramier M.
Sci Rep. (2017) 7:41026.

A FRET biosensor reveals spatiotemporal activation and functions of Aurora kinase A in living cells.
Bertolin G, Sizaire F, Herbomel G, Reboutier D, Prigent C, Tramier M.
Nat Commun. (2016) 7:12674.

Quantitative study of protein-protein interactions in live cell by dual-color fluorescence correlation spectroscopy.
Padilla-Parra S, Audugé N, Coppey-Moisan M, Tramier M.
Methods Mol Biol. 2014.