The long-read sequencing technology proposed by ONT (Oxford Nanopore Technologies) is based on the detection of a differential current as a single linear molecule passes through a biological nanopore anchored in a synthetic membrane. The nanopore is an ion flow sensor that varies according to the steric hindrance of the compound passing through it, enabling the identification of the sequence of a DNA strand over a long length (up to several Mb), whatever its composition in terms of repeated elements, GC-rich or methylated regions.
