Gilles Salbert Group (SP@RTE)

The genome is used to build complex organisms made of various tissues and cell types, each harbouring unique properties depending on specific gene activities. Within the nucleus, the genome is organized as chromatin, a fiber made of DNA and proteins. We develop a multi-scale approach to analyse the spatio-temporal dynamics of the chromatin architecture in relation with the control of gene activity.

Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling.
Longarini EJ, Dauben H, Locatelli C, Wondisford AR, Smith R, Muench C, Kolvenbach A, Lynskey ML, Pope A, Bonfiglio JJ, Pinto Jurado E, Fajka-Boja R, Colby T, Schuller M, Ahel I, Timinszky G, O'Sullivan RJ, Huet S*, Matic I*.
Mol Cell, May 2023.

HPF1-dependent histone ADP-ribosylation triggers chromatin relaxation to promote the recruitment of repair factors at sites of DNA damage.
Smith R#*, Zentout S#, Rother M, Bigot N, Chapuis C, Mihuț A, Zobel FF, Ahel I, van Attikum H, Timinszky G*, Huet S*.
Nat Struct Mol Biol, Apr 2023.

Identification of key residues of the DNA glycosylase OGG1 controlling efficient DNA sampling and recruitment to oxidized bases in living cells.
D'Augustin O, Gaudon V, Siberchicot C, Smith R, Chapuis C, Depagne J, Veaute X, Busso D, Di Guilmi AM, Castaing B, Radicella JP, Campalans A*, Huet S*.
Nucleic Acid Res, Apr 2023.

TET2-mediated epigenetic reprogramming of breast cancer cells impairs lysosome biogenesis.
Laurent A, Madigou T, Bizot M, Turpin M, Palierne G, Mahé E, Guimard S, Métivier R, Avner S, Le Péron C*, Salbert G*.
Life Sci. Alliance, Mar 2022.

The N-terminal domain of TET1 promotes the formation of dense chromatin regions refractory to transcription.
Lejart A, Zentout S, Chapuis C, D'Augustin O, Smith R, Salbert G*, Huet S*.
Chromosoma, Mar 2022.

Label-free microscopy of mitotic chromosomes using the polarization orthogonality breaking technique.
Desapogu R, Le Marchand G, Smith R, Ray P, Gillier E, Dutertre S, Alouini M, Tramier M, Huet S*, Fade J*.
Biomed Opt Express. 2021 Jul.

The chromatin remodeler ALC1 underlies resistance to PARP inhibitor treatment.
Juhász S#, Smith R#, Schauer T, Spekhardt D, Mamar H, Zentout S, Chapuis C, Huet S*, Timinszky G*
Science Advances 2020 Dec. 

Lost in the Crowd: How Does Human 8-Oxoguanine DNA Glycosylase 1 (OGG1) Find 8-Oxoguanine in the Genome?
D'Augustin O, Huet S*, Campalans A*, Radicella JP*.
Int J Mol Sci. 2020 Nov. 

Poly(ADP-ribose)-dependent chromatin unfolding facilitates the association of DNA-binding proteins with DNA at sites of damage. 
Smith R, Lebeaupin T, Juhasz S, Chapuis C, D'Augustin O, Dutertre S, Burkovics P, Biertumpfel C, Timinszky G*, Huet S*.
Nucleic Acid Res. 2019 Sept.

The hydroxymethylome of multiple myeloma identifies FAM72D as a 1q21 marker linked to proliferation. 
Chatonnet F, Pignarre A, Sérandour AA, Caron G, Avner S, Robert N, Kassambara A, Laurent A, Bizot M, Agirre X, Prosper F, Martin-Subero JI, Moreaux J, Fest T*, Salbert G*.
Haematologica 2019 Jun.

CHD3 and CHD4 recruitment and chromatin remodeling activity at DNA breaks is promoted by early poly(ADP-ribose)-dependent chromatin relaxation.
Smith R, Sellou H, Chapuis C, Huet S*, Timinszky G*.
Nucleic Acids Res. 2018 May.

Cytosine modifications modulate the chromatin architecture of transcriptional enhancers. 
Mahé EA, Madigou T, Sérandour AA, Bizot M, Avner S, Chalmel F, Palierne G, Métivier R, Salbert G.
Genome Res. 2017 Apr.

Inactivation of the Nuclear Orphan Receptor COUP-TFII by Small Chemicals. 
Le Guével R, Oger F, Martinez-Jimenez CP, Bizot M, Gheeraert C, Firmin F, Ploton M, Kretova M, Palierne G, Staels B, Barath P, Talianidis I, Lefebvre P, Eeckhoute J, Salbert G.
ACS Chem. Biol. 2017 Jan.

The poly(ADP-ribose)-dependent chromatin remodeler Alc1 induces local chromatin relaxation upon DNA damage.
Sellou H, Lebeaupin T, Chapuis C, Smith R, Hegele A, Singh HR, Kozlowski M, Bultmann S, Ladurner AG, Timinszki G*, Huet S*.
Mol Biol Cell 2016 Oct.

Changes in gene expression and Estrogen Receptor cistrome in mouse liver upon acute E2 treatment.
Palierne G, Fabre A, Solinhac R, Le Péron C, Avner S, Lenfant F, Fontaine C, Salbert G, Flouriot G, Arnal JF, Métivier R.
Mol Endocrinol. 2016 May.

Single-CpG resolution mapping of 5-hydroxymethylcytosine by chemical labeling and exonuclease digestion identifies evolutionarily unconserved CpGs as TET targets.
Sérandour A.A, Avner S, Mahé E.A, Madigou T, Guibert S, Weber M, Salbert G. 
Genome Biol. 2016 Mar.

Cell cycle-dependent reconfiguration of the DNA (hydroxy)methylome during terminal differentiation of human B cells into plasma cells. 
Caron G, Hussein M, Kulis M, Delaloy C, Chatonnet F, Pignarre A, Avner S, Lemarié M, Mahé E, Verdaguer-Dot N, Queirós AC, Tarte K, Martín-Subero JI, Salbert G*, Fest T*. 
Cell Reports. 2015 Oct. -

Dynamic estrogen receptor interactomes control estrogen-responsive trefoil Factor (TFF) locus cell-specific activities.
Quintin J, Le Péron C, Palierne G, Bizot M, Cunha S, Sérandour AA, Avner S, Henry C, Percevault F, Belaud-Rotureau MA, Huet S, Watrin E, Eeckhoute J, Legagneux V, Salbert G, Métivier R. 
Mol Cell Biol. 2014 Jul.

Crowded chromatin is not sufficient for heterochromatin formation and not required for its maintenance. 
Walter A, Chapuis C, Huet S*, Ellenberg J*.
J Struct. Biol. 2013 Dec. -

Dynamic hydroxymethylation of deoxyribonucleic acid marks differentiation-associated enhancers. 
Sérandour AA, Avner S, Oger F, Bizot M, Percevault F, Lucchetti-Miganeh C, Palierne G, Gheeraert C, Barloy-Hubler F, Péron CL, Madigou T, Durand E, Froguel P, Staels B, Lefebvre P, Métivier R, Eeckhoute J, Salbert G. 
Nucleic Acids Res. 2012 Sep.

Epigenetic switch involved in activation of pioneer factor FOXA1-dependent enhancers.
Sérandour AA, Avner S, Percevault F, Demay F, Bizot M, Lucchetti-Miganeh C, Barloy-Hubler F, Brown M, Lupien M, Métivier R, Salbert G*, Eeckhoute J*. 
Genome Res. 2011 Apr.

Cyclical DNA methylation of a transcriptionally active promoter. 
Métivier R, Gallais R, Tiffoche C, Le Péron C, Jurkowska RZ, Carmouche RP, Ibberson D, Barath P, Demay F, Reid G, Benes V, Jeltsch A, Gannon F, Salbert G.  
Nature. 2008 Mar.

* Corresponding authors,  # co-first authors