Email : denis [dot] chretien [at] univ-rennes [dot] fr

Phone : +33 (0)2 23 23 67 64

MiToS team members


Université de Rennes 1
UMR 6290, MiToS team
Campus de Beaulieu, Bât. 13, Pièce 215
35042 Rennes Cedex

In a few words

Denis Chrétien performed his PhD at the Center for Atomic Research in Grenoble (1987-90), under the supervision of Richard H. Wade. He then joined the European Molecular Biology Laboratory in Heidelberg to perform a post-doctorate in the groups of Eric Karsenti and Steve Fuller (1991-96). In 1996, he obtained a position at the interface between Cell Biology, Structural Biology and Biophysics, under the supervision of Anthony Hyman (1996-98). In 1998, Denis Chrétien was recruited as a CNRS scientist at the University of Tours (1998-99), and then moved to Rennes under the ATIPE program from the CNRS (first ATIPE in Rennes), at the Cell and Molecular Interaction Unit (1999-1012). In 2012, the team joined the Institute of Genetics and Development of Rennes.


Scientific manager of the MRic TEM-Molecular platform.

Selected publications

Characterization of Microtubule Lattice Heterogeneity by Segmented Subtomogram Averaging
Bousquet C, Heumann J, Chrétien D, Guyomar C.
Bio Protocol 2023 Feb.

The microtubule lattice: a brief historical perspective
Chrétien D, Guyomar C
Biology of the Cell 2023 Feb.

Changes in seam number and location induce holes within microtubules assembled from porcine brain tubulin and in Xenopus egg cytoplasmic extracts
Guyomar C, Bousquet C, Ku S, Heumann J, Guilloux G, Gaillard N, Heichette C, Duchesne L, Steinmetz M. O, Gibeaux R, Chrétien D
Elife 2022 Dec.

Structural model for differential cap maturation at growing microtubule ends 
Estévez-Gallego J*, Josa-Prado F*, Ku S*, Buey RM, Balaguer FA, Prota AE, Lucena-Agell D, Kamma-Lorger C, Yagi T, Iwamoto H, Duchesne L, Barasoain I, Steinmetz MO, Chrétien D, Kamimura S, Díaz JF, Oliva MA
Elife 2020 Mar.
*Co-first authors

Lattice defects induce microtubule self-renewal
Schaedel L., Triclin S., Chrétien D., Abrieu A., Aumeier C., Gaillard J., Blanchoin L., Théry M., John K.
Nature Physics, 2019, Jan.

EB1 interacts with outwardly curved and straight regions of the microtubule lattice
Guesdon A*, Bazile F*, Buey RM, Mohan R, Monier S, Rodríguez García R, Angevin M, Heichette C, Wieneke R, Tampé R, Duchesne L, Akhmanova A, Steinmetz MO, Chrétien D
Nat Cell Biol. 2016, Sep.
*Co-first authors

Single versus dual-axis cryo-electron tomography of microtubules assembled in vitro: Limits and perspectives.
Guesdon A, Blestel S, Kervrann C, Chrétien D.
J Struct Biol. 2013 Feb.

EB1 regulates microtubule dynamics and tubulin sheet closure in vitro.
Vitre B, Coquelle FM, Heichette C, Garnier C, Chrétien D, Arnal I. 
Nat Cell Biol. 2008 Apr.

Microtubules switch occasionally into unfavorable configurations during elongation.
Chrétien D, Fuller SD.
J Mol Biol, 2000, May.

Structural changes at microtubule ends accompanying GTP hydrolysis: information from a slowly hydrolyzable analogue of GTP, guanylyl (α,β)methylenediphosphonate.
Müller-Reichert T, Chrétien D, Severin F, Hyman AA.
Proc Natl Acad Sci U S A, 1998 Mar.

Structure of growing microtubule ends: two-dimensional sheets close into tubes at variable rates.
Chrétien D, Fuller SD, Karsenti E.
J Cell Biol, 1995, Jun.